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Image Search Results
Journal: RNA Biology
Article Title: Global assessment of GU-rich regulatory content and function in the human transcriptome
doi: 10.4161/rna.8.4.16283
Figure Lengend Snippet: GRE- and ARE-motif effect on mRNA stability Microarray-based mRNA half-life data (see Materials and Methods for details) were stratified according to the experimental condition, transcript type and half-life classification (short vs. long half-lives, based upon a Gaussian-mixture model fit. (A) The histograms of log-2 values (columns) of mRNA half-lives in each gene category (Control, ARE-genes and GRE-genes) were obtained and fitted using a bi-modal Gaussian mixture model (line). The control set represent mRNAs with neither ARE nor GRE. A representative model under the anti-CD3 experimental condition is shown. (B) The fraction of short half-life transcripts out of the total within each stratum. Data are Mean ± SEM and represent fitted parameter uncertainties. For each stimulus, ARE-mRNAs and GRE-mRNAs results were compared with the Control using the unpaired t-test with Bonferroni correction for multiple testing. **p < 0.01 and *p < 0.05. Using the 2-way ANOVA test, the effect of the motif (Control, ARE+ or GRE+) was found to be significant at p < 0.01. (C) A simpler classification scheme based on the arbitrary cutoff of 64 (26) minutes to classify short and long half-life transcripts yields essentially the same results as in (A). (D) The proportions of control mRNAs, ARE-mRNAs and GRE-mRNAs that do not change their stability patterns (non-switching class). p < 0.001 using chi-square analysis.
Article Snippet: We proceeded to estimate the ratio of the short set to the long set by fitting the histogram data with a
Techniques: Microarray, Control